Structured Bilayer Biomimetic Organoids: A Platform to Study Hormonal and Tumorigenic Events in the Mammary Gland
Poster #: 123
Session/Time: B
Author:
Arielle Wolter, BS, MS
Mentor:
Patrick Sachs, BS, PhD
Research Type: Basic Science
Abstract
INTRODUCTION:
The microenvironment of the mammary gland is dynamic in its ability to change over the lifetime of an organism. This includes ductal luminal epithelial and myoepithelial cellular fate and physiological structure necessary for the development of healthy tissues (Inman et al., 2015). Recent advances have shown the effects of the microenvironment on cell fate include differentiation of cell types (Bruno et al., 2017). Current models have yet to produce a structurally accurate and biochemically representative model that can simulate mammary function of the body in vivo. Therefore, we purpose a biomimetic mammary gland model that mimics this niche environment in structure and function.
METHODS:
To achieve this here we test the combined use of mammary derived ECM with 3D resin printing assisted production of a structural platform to provide consistent growth of mammary ductal organoids. We hypothesize the system will influence primary cell fate through biochemical factors and structural components to a mammary gland bilayer state encompassing myoepithelial and epithelial tissues and increase precision of cell placement and greater control of growth when incorporated into our 3D bioprinting system. These in vitro glands will better replicate a mammary gland and provide a superior platform for the study of mammary gland dynamics and tumorigenesis.
RESULTS:
Primary cells have been successfully reprogrammed and influenced to a mammary stem cell linage. Preliminary results in have shown successful structure orientation and compatibility of mammary type stem cells in alginate stabilized collagen.
The microenvironment of the mammary gland is dynamic in its ability to change over the lifetime of an organism. This includes ductal luminal epithelial and myoepithelial cellular fate and physiological structure necessary for the development of healthy tissues (Inman et al., 2015). Recent advances have shown the effects of the microenvironment on cell fate include differentiation of cell types (Bruno et al., 2017). Current models have yet to produce a structurally accurate and biochemically representative model that can simulate mammary function of the body in vivo. Therefore, we purpose a biomimetic mammary gland model that mimics this niche environment in structure and function.
METHODS:
To achieve this here we test the combined use of mammary derived ECM with 3D resin printing assisted production of a structural platform to provide consistent growth of mammary ductal organoids. We hypothesize the system will influence primary cell fate through biochemical factors and structural components to a mammary gland bilayer state encompassing myoepithelial and epithelial tissues and increase precision of cell placement and greater control of growth when incorporated into our 3D bioprinting system. These in vitro glands will better replicate a mammary gland and provide a superior platform for the study of mammary gland dynamics and tumorigenesis.
RESULTS:
Primary cells have been successfully reprogrammed and influenced to a mammary stem cell linage. Preliminary results in have shown successful structure orientation and compatibility of mammary type stem cells in alginate stabilized collagen.