Imaging & Irradiation
The Center provides essential training for all instruments and technical support for troubleshooting. We also provide consultation, hands-on service on experimental design and data analysis. For more info regarding services and rates contact our Core Facility Supervisor Dr. Yu (Ellen) Jing.
Leica inverted microscope
The Leica DMi8 system is a high profile inverted fluorescent microscope capable of conducting X, Y, Z and T 4-dimentional imaging for up to four fluorescent channels with representative fluorophores DAPI, FITC, Cy3 and Cy5. The "Navigator" function allows large area scanning and imaging at the specified magnification via an internal auto-stitching process. Time lapse imaging is available and autofocus is optional. Objectives range from 5x to 40x and the 5x, 10x and 20x objectives are long working distance ones meaning that they are good for imaging both slides as well as common culture vessels.
Resources
https://www.leica-microsystems.com/products/light-microscopes/p/leica-dmi8/
Laser Doppler System
The LDI2 laser Doppler system, manufactured by Moor Instruments, offers a non-invasive solution to blood flow measurement using the infrared laser light to facilitate research applications on study of angiogenesis and reperfusion. The spatial resolution and the scan area, which are inversely related, can reach up to 100 micron and 50 cm x 50 cm, respectively.
Resources
https://www.moor.co.uk/en-us/products/imaging/laser-doppler-imaging/
IV100 Fluorescent Microscopy System
The Olympus IV100 microscopy system provides a tool for users to image cellular and subcellular changes in tissues. The system is equipped with four lasers 488, 561, 633 and 748nm and a position-adjustable scanhead for up to an X,Y,Z,T 4-dimensional scan.
Resources
http://www.olympus114.com/ctlg/IV100.pdf
IVIS Spectrum
The IVIS spectrum marketed by Caliper, a PerkinElmer company for non-invasive imaging of tissues is equipped with 10 excitation filters and 18 detection filters spanning 430-850 nm in wavelength. It also has two detection modes, epi- and trans- for detection of fluorescent/bioluminescent light in shallow and deep tissues, respectively.
Resources
https://www.perkinelmer.com/product/ivis-instrument-spectrum-120v-andor-c-124262
X-ray Irradiator
The X-ray irradiator, manufactured by RadSource, provides stable and uniform irradiation at the intensity of 0.01-95Gr/min for the duration of 1-9999 seconds in a highly safe manner.
Resources
https://radsource.com/wp-content/uploads/RS-2000-Brochure.pdf