Olga Pakhomova

Ph.D. in Radiation Biology/ Biophysics, Medical Radiology Research Center, (1995)

M.S. in Microbiology, Moscow State University, (1982)

Research Interests

The current research is centered on understanding and characterization of cell response to pulsed electric fields treatments, with particular emphasis on nanosecond-duration pulses and cell death mechanisms.Main topics of the research include:1) mechanisms of the PEF-triggered cell death, characterization of the cell death pathways2) escalation of cell sensitivity to pulsed electric field (PEF) for advancement of PEF-mediated tissue and tumor ablation3) PEF-induced communication between the cells

Articles

Semenov, I., Grigoryev, S., Neuber, J. U., Zemlin, C., Pakhomova, O., Casciola, M. and Pakhomov, A. (2018). Excitation and injury of adult ventricular cardiomyocytes by nano- to millisecond electric shocks. Scientific Reports 8 , pp. 12.

Muratori, C., Pakhomov, A., Gianulis, E., Meads, J., Casciola, M., Mollica, P. A. and Pakhomova, O. (2017). Activation of the phospholipid scramblase tmem16f by nanosecond pulsed electric field (nspef) facilitates its diverse cytophysiological effects. Journal of Biological Chemistry.

Muratori, C., Pakhomov, A. and Pakhomova, O. (2017). Effect of cooling on cell volume and viability after nanoelectroporation. The Journal of Membrane Biology 250 (2) , pp. 217-224.

Pakhomova, O., Gregory, B. W.. and Pakhomov, A. (2013). Facilitation of electroporative drug uptake and cell killing by electrosensitization. Journal of Cellular and Molecular Medicine 17 (1) , pp. 154-159.

Semenov, I., Xiao, S., Pakhomova, O. and Pakhomov, A. (2013). Recruitment of the intracellular Ca2+ by ultrashort electric stimuli: The impact of pulse duration. Cell Calcium 54 (3) , pp. 145-150.

Pakhomova, O., Gregory, B. W.., Semenov, I. and Pakhomov, A. (2013). Two Modes of Cell Death Caused by Exposure to Nanosecond Pulsed Electric Field. Plos One 8 (7).

Pakhomova, O., Khorokhorina, V. A.., Bowman, A. M.., Rodaite-Riseviciene, R., Saulis, G., Xiao, S. and Pakhomov, A. (2012). Oxidative effects of nanosecond pulsed electric field exposure in cells and cell-free media. Archives of Biochemistry and Biophysics 527 (1) , pp. 55-64.

Ibey, B. L.., Roth, C. C.., Pakhomov, A., Bernhard, J. A.., Wilmink, G. J.. and Pakhomova, O. (2011). Dose-Dependent Thresholds of 10-ns Electric Pulse Induced Plasma Membrane Disruption and Cytotoxicity in Multiple Cell Lines. Plos One 6 (1).

Pakhomova, O., Gregory, B. W.., Khorokhorina, V. A.., Bowman, A. M.., Xiao, S. and Pakhomov, A. (2011). Electroporation-Induced Electrosensitization. Plos One 6 (2).

Nesin, O. M.., Pakhomova, O., Xiao, S. and Pakhomov, A. (2011). Manipulation of cell volume and membrane pore comparison following single cell permeabilization with 60-and 600-ns electric pulses. Biochimica Et Biophysica Acta-Biomembranes 1808 (3) , pp. 792-801.

Bowman, A. M.., Nesin, O. M.., Pakhomova, O. and Pakhomov, A. (2010). Analysis of Plasma Membrane Integrity by Fluorescent Detection of Tl+ Uptake. Journal of Membrane Biology 236 (1) , pp. 15-26.

Pakhomova, O., Taylor, A. B.., Becker, A., Holloway, S. P.., Kannan, T. R.., Baseman, J. B.. and Hart, P. J.. (2010). Crystallization of community-acquired respiratory distress syndrome toxin from Mycoplasma pneumoniae. Acta Crystallographica Section F-Structural Biology and Crystallization Communications 66 , pp. 294-296.

Pakhomov, A., Bowman, A. M.., Ibey, B. L.. and Pakhomova, O. (2010). Nanopore Formation in Cells Exposed to Nanosecond Electric Pulses. Biophysical Journal 98 (3) , pp. 375a-375a.

Ibey, B. L.., Pakhomov, A., Gregory, B. W.., Khorokhorina, V. A.., Roth, C. C.., Rassokhin, M., Bernhard, J. A.., Wilmink, G. J.. and Pakhomova, O. (2010). Selective cytotoxicity of intense nanosecond-duration electric pulses in mammalian cells. Biochimica Et Biophysica Acta-General Subjects 1800 (11) , pp. 1210-1219.

Pakhomov, A., Bowman, A. M.., Ibey, B. L.., Andre, F. M.., Pakhomova, O. and Schoenbach, K. H. (2009). Lipid nanopores can form a stable, ion channel-like conduction pathway in cell membrane. Biochemical and Biophysical Research Communications 385 (2) , pp. 181-186.

Pakhomov, A., Ibey, B. L.., Bowman, A. M.., Andre, F. M.. and Pakhomova, O. (2009). Nanosecond-Duration Electric Pulses Open Nanometer-Size Pores in Cell Plasma Membrane. World Congress on Medical Physics and Biomedical Engineering, Vol 25, Pt 13 25 (13) , pp. 17-20.

Ilangovan, U., Bhuiyan, S. H.., Hinck, C. S.., Hoyle, J. T.., Pakhomova, O., Zwieb, C. and Hinck, A. P.. (2008). A-Fulgidus SRP54 m-domain. Journal of Biomolecular Nmr 41 (4) , pp. 241-248.

Pakhomov, A., Shevin, R., White, J. A.., Kolb, J. F., Pakhomova, O., Joshi, R. and Schoenbach, K. H. (2007). Membrane permeabilization and cell damage by ultrashort electric field shocks. Archives of Biochemistry and Biophysics 465 (1) , pp. 109-118.

Walker, K., Pakhomova, O., Kolb, J. F., Schoenbach, K. H., Stuck, B. E.., Murphy, M. R.. and Pakhomov, A. (2006). Oxygen enhances lethal effect of high-intensity, ultrashort electrical pulses. Bioelectromagnetics 27 (3) , pp. 221-225.

Zuniga, J. E.., Groppe, J. C.., Cui, Y. M.., Hinck, C. S.., Contreras-Shannon, V., Pakhomova, O., Yang, J. H.., Tang, Y. P.., Mendoza, V., Lopez-Casillas, F., Sun, L. Z.. and Hinck, A. P.. (2005). Assembly of T beta RI : T beta RII : TGF beta ternary complex in vitro with receptor extracellular domains is cooperative and isoform-dependent. Journal of Molecular Biology 354 (5) , pp. 1052-1068.

Pakhomova, O., Deep, S., Huang, Q. J.., Zwieb, C. and Hinckl, A. P.. (2002). Solution structure of protein SRP19 of Archaeoglobus fulgidus signal recognition particle. Journal of Molecular Biology 317 (1) , pp. 145-158.

Bhuiyan, S. H.., Pakhomova, O., Hinck, A. P.. and Zwieb, C. (2001). Complexes with truncated RNAs from the large domain of Archaeoglobus fulgidus signal recognition particle. Fems Microbiology Letters 198 (2) , pp. 105-110.

Pakhomova, O., Cui, Y., Zwieb, C. and Hinck, A. P.. (2001). Sequence-specific H-1, C-13 and N-15 signal assignments and secondary structure of Archaeoglobus fulgidus SRP19. Journal of Biomolecular Nmr 20 (2) , pp. 187-188.

Pakhomova, O., Yeh, L. C. C.., Monette, J. and Lee, J. C.. (1999). In vitro assembly of yeast 5S rRNA and a fusion protein containing ribosomal protein L5 and maltose binding protein. Biochimie 81 (11) , pp. 1015-1023.

Pakhomov, A., Akyel, Y., Pakhomova, O., Stuck, B. E.. and Murphy, M. R.. (1998). Current state and implications of research on biological effects of millimeter waves: A review of the literature. Bioelectromagnetics 19 (7) , pp. 393-413.

Pakhomova, O., Belt, M. L.., Mathur, S. P.., Lee, J. C.. and Akyel, Y. (1998). Ultra-wide band electromagnetic radiation does not affect UV-induced recombination and mutagenesis in yeast. Bioelectromagnetics 19 (2) , pp. 128-130.

Pakhomova, O., Pakhomov, A. and Akyel, Y. (1997). Effect of millimeter waves on UV-induced recombination and mutagenesis in yeast. Bioelectrochemistry and Bioenergetics 43 (2) , pp. 227-232.

Pakhomova, O., Belt, M. L.., Mathur, S. P.., Lee, J. C.. and Akyel, Y. (1997). Lack of genetic effects of ultrawide-band electromagnetic radiation in yeast. Electro- and Magnetobiology 16 (3) , pp. 195-201.

Tsyb, T. S.., Selyova, N. G.., Kabakova, N. M.., Myasnik, M. N.. and Pakhomova, O. (1987). Contribution of a Photoreactivable Component to the Oxygen Effect Observed in Certain Rad Mutants of Saccharomyces-Cerevisiae after Exposure to High-Energy Electrons. International Journal of Radiation Biology 51 (4) , pp. 655-664.