Biological Sciences

Barbara Hargrave

Suite 300 Research Park Ii 4211 Monarch Way
Norfolk, 23529

B.S. in , Hampton University, ()

Ph.D. in Physiology/Pharmacology, Bowman Gray School Of Medicine, Wake Forest University, (1985)

Contracts, Grants and Sponsored Research

Hargrave, B. "Platelet Activation Using Nanosecond Pulse Electric Fields" $80000. Old Dominion University. January, 2007 - ,
Osgood, C. J. "Development of a mouse model to investigate gender differences in drug-induced cardiovascular disease" $30000. State. , 2004 - , 2005
Hargrave, B. "Jeffers Foundation Grant" $30000. . , 2004 - , 2004


Use of Platelet Gel for the treatment of Cardiac Ischemia and Reperfusion Injury

Research Interests

Cardiovascular Physiology and Pharmacology


Hargrave, B. (2016). Nanosecond Pulsed Platelet-Rich Plasma (nsPRP) Improves Mechanical and Electrical Cardiac Function Following Myocardial Reperfusion Injury. Physiological Reports.
Eldelblute, C. (2015). Donor platelet plasma components inactivate sensitive and multidrug resistant Acinetobacter baumannii isolates. Pathog Dis 73.
Edelbute, C. (2015). Human platelet gel supernatant inactivates opportunistic wound pathogens on skin. . Platelets 26.
Basu, G. (2014). Prevention of distal flap necrosis in a rat random flap model by gene electrotransfer delivering VEGF165 plasmid.. J Gene Medicine 16 , pp. 55-65.
Hargrave, B. (2014). Gene Electro transfer of plasmid encoding VEGF Improves pVEGF expression and Perfusion In the Ischemic Swine Heart. PLsoOne.
Hargrave, B. (2012). Electroporation-mediated gene transfer directly to the swine heart.. Gene Therapy 20.
Zhang, J. (2008). Nanosecond Pulse Electric Field (nanopulse): a Novel Non-ligand Agonist for Platelet Activation. Archives of Biochemistry and Biophysics 471 , pp. 240-248.
Osgood, C. J. (2007). Bioelectric effects of intense nanosecond pulses. IEEE Transact Dielectrics Electrical Insul 14 , pp. 1088-1109.
Schoenbach, K. H. (2007). Bioelectric Effects of Nanosecond Pulses. IEEE Trans. Diel. Electr. Insul. 14 (5) , pp. 1088-1109.
Tiangco, D. A. (2005). 3,4-Methylendioxymethamphetamine activates nuclear factor-kappaB, increases intracellular calcium, and modulates gene transcription in rat heart cells. Cardiovasc Toxicol 5 (3) , pp. 301-310.
Osgood, C. J. (2005). Cocaine increases intracellular calcium and reactive oxygen species, depolarizes, mitochondria and activates genes associated with heart failure and remodeling. Cardiovasc Toxicol 5 (4) , pp. 377-390.
Hargrave, B. (2003). Cocaine but not morphine causes the generation of reactive oxygen species and activation of NFκB in transiently co-transfected heart and renal epithelial cells. Cardiovascular Toxicology 3 , pp. 141-151.
Hargrave, B. (2002). Cocaine activates the renin angiotensin system in pregnant rabbits and alters the response to ischemia. Cardiovascular Toxicology 2 , pp. 91.
Hargrave, B. (1995). Effects of phenylephrine induced increase in arterial pressure and closure of the ductus arteriosus on the secretion of atrial natriuretic peptide and renin in the ovine fetus. Life Sci 57 , pp. 31.
Hargrave, B. (1995). Intrauterine exposure to cocaine increased plasma ANP but did not alter hypoxanthine concentrations in the sheep fetus. Life Sci 56 , pp. 1689.
Tkacs, N. C. (1990). Pharmacological evidence for involvement of the sympathetic nervous system in the increase in renin secretion produced by a low sodium diet in rats. Life Sci 47 , pp. 2317.
Hargrave, B. (1990). Pulmonary vascular effects of exogenous atrial natriuretic peptide in sheep fetuses. Ped Res 27 , pp. 140.
Hargrave, B. (1989). Renal and cardiovascular effects of atrial natriuretic peptide in the sheep fetus. Pediatric Res 26 , pp. 1.
Hargrave, B. (1986). By 95 days of gestation CRF increases plasma ACTH and cortisol in ovine fetuses. Am J Physiol 250 , pp. E422.
Hargrave, B. (1985). By 94 days of gestation plasma cortisol increases block ACTH response of hypotension in lamb fetuses. Am J Physiol 249 , pp. E350.
Jones, C. M. (1985). Catecholamine responses in fetal lambs subjected to hemorrhage. Am J Obstet Gynecol 151 , pp. 475.
Rose, J. C. (1985). Corticotrophin-releasing factor induced adrenocorticortropic hormone release in the sheep fetus: blockade by cortisol. Am J Obstet Gynecol 151 , pp. 1128.
Dix, P. M . (1984). Cortisol infusion blocks adrenocorticortropic hormone but not vasopressin responses to hypotension in fetal lambs. Am J Obstet Gynecol 148 , pp. 317.
Rose, J. C. (1983). A vasopressin antagonist block norepinephrine and epinephrine responses to hemorrhage in the fetus. Endocrinol 113 , pp. 2314.
Kelly, R. T. (1983). Vasopressin is important for restoring cardiovascular homeostasis in fetal lambs subjected to hemorrhage. Am J Obstet Gynecol 146 , pp. 807.


Hargrave, B. (September , 2009). An Alternative To The use of Thrombin In The Activation of Platelet Gel: Nanosecond Pulsed Electric Fields (nsPEFs) Perfusionist Society Meeting Greensboro, NC.
Hargrave, B. ( 2008). Alternative Method for Platelet Activation In the Preparation of Platelet Gel International Platelet Gel and Stem Cell Symposium Fort Myers, FL.
Hargrave, B. ( 2008). A Novel Approach for Wound Healing: Platelet Activation Using Nanosecond Pulse Electric Fields Molecular Medicine Seminar Series Norfolk, VA.
Hargrave, B. ( 2004). Cocaine and the Cardiovascular System Research Day Norfolk, VA.
Hargrave, B. ( 2003). Cocaine and the Cardiovascular System Revisited: Still a Deadly Combination Norfolk, VA.
Hargrave, B. ( 2002). Cocaine Decreases Heart Function, Causes the Production of Reactive Oxygen Species, and Activates NFκB Heart Failure and Circulatory Summit I Cleveland, OH.
Hargrave, B. ( 2001). Effect of Cocaine and Ecgonine Methyl Ester on Plasma Renin Activity and Heart Function in Pregnant and Nonpregnant Rabbits and on NF-κB Rel Transcription Factors Annual Meeting Orlando, FL.
Hargrave, B. ( 2001). Effect of Cocaine and Ecgonine Methyl Ester on the Renin Angiotensin System and Cardiovascular Function in Pregnant, Nonpregnant, and Male Rabbits FSEBE Meeting Orlando, FL.
Hargrave, B. ( 2000). Cocaine and the Cardiovascular System: A Deadly Combination Norfolk, VA.
Jordan, T. ( 1999). Cardiovascular Effects of Cocaine in the Pregnant Rabbit Norfolk, VA.
Parson, H. ( 1999). Ion Mobility Spectrometry of Animals Exposed to Physiologically Active Substances Chemical and Biological Detection Poster Presentation Session Newport, RI.
Parson, H. ( 1998). Ion Mobility Spectrometry with Samples of Biological Origin 76th Annual Meeting Fairfax, VA.
Parson, H. ( 1998). Ion Mobility Spectrometry with Animal Tissues and Plasma Multi-Discipline Student Research Poster Session Norfolk, VA.
Chambliss, A. ( 1998). The Effect of Heroin on Plasma Renin Activity and ANP Secretion Multi-Discipline Student Research Poster Session Norfolk, VA.
Hargrave, B. ( 1995). Effects of Increased Afterload on ANP Secretion in the Developing Ovine Fetus Burlington, VT.
Hargrave, B. ( 1994). Renal and Pulmonary Effects of ANP in the Developing Ovine Fetus Washington, D.C..
Hargrave, B. ( 1993). Renal & Pulmonary Effects of ANP in the Developing Ovine Fetus Norfolk, VA.
Hargrave, B. ( 1992). Mechanism of Action of Cocaine on the Cardiovascular System of the Developing Fetus Research in Progress II: Women's Issues Norfolk, VA.
  • 1997: Hugo A. Owens Outstanding Faculty Award, Old Dominion University
  • 1997: Outstanding Service Award, Old Dominion University
  • 1987: Neuroscience Travel Award,
  • 1987: Travel Award, American Physiological Society
  • 1986: President's Fellowship, University of California, San Francisco