November 9, 2001 3:00 pm (Friday)
|
Prof. X. Nancy Xu Old Dominion University Chemistry Department
Single-Molecule Studies of Single Living Cells
A new field of scientific research has emerged in recent years, which focuses on study of single molecules. Single-molecule detection is a way to study and characterize detailed physical and chemical properties of individual molecules. This approach allows one to look beyond the ensemble average, testing of fundamental principles and may lead to new paradigms of our thinking, and technological and methodological developments with application in a variety of research fields (e.g., medicine, biotechnology, molecular biology, material). Single living cells assemble the wide spectra of molecules and ions in a tiny compartment (~10-mm in diameter), display diverse physical and chemical reactions at all time scales and possess the most powerful and intelligent micro/nano-machinery. Therefore, single-molecule studies of single living cells present unique opportunities to overcome such overwhelming ensemble average and allow individual molecule and reaction to be singled out and explored in details for advancing the understanding of cellular function and nano-environment in complex biosystems. In this seminar, our recent development of nanoparticle probes and single-molecule dynamics microscopy for real-time monitoring of single ligand-receptor interactions on single living eukaryote cells and real-time probing of membrane pump machinery of single living bacteria cells will be discussed. |