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- Michael Stacey
- Research Associate Professor
- Frank Reidy Center for Bioelectrics
- 300 4211 Monarch Way
- BS in Zoology and PhD in cancer studies at the University of Hull and University of Birmingham, England. Fellowships at the University of Birmingham, and Oxford, England, before coming to EVMS in 1997.
Moved to the Center for Bioelectrics ODU in 2007.
- University Of Birmingham,
Major: Cancer Biology
Degree: Ph. D.
- Research interests.
My research interests are in the area of Biomedicine, understanding the causes of disease and their underlying biology.
1). Pectus excavatum is a common inherited chest wall deformity where the sternum is depressed towards the spinal column, compressing the chest and affecting heart and lung function. The underlying cause is thought to be due to mechanical weakness in the chest cartilage, likely due to abnormal protein deposition or interaction. The mechanical properties of cartilage closely correlate with the electrical properties, and therefore we expect both properties to be compromised in affected costal cartilages. The internal electrical charge of cartilage is responsible for influx of solutes, which gives cartilage both strength and pliability. We are investigating changes in electrical and mechanical properties of proteins in individuals with pectus excavatum as potential underlying causes. We consider it artificial to separate mechanical responses from electrical responses in this biological tissue, as one is very dependent upon the other. This disorder is a model for uniting biomechanics and bioelectrics with the advantages of patient benefit.
2). Carbon nanotubes are finding increased use in biological and medical applications. They possess unique physical, chemical and electrical properties that could be utilized in the treatment of disease. Electrically, carbon nanotubes are a basic building block for nanometer scaled electronic structures and have the world's highest conductivity per unit mass. Although there are many conflicting reports regarding the toxicity of carbon nanotubes, the design of carbon nanotubes for specific biological applications hold great promise. The sensitivity of carbon nanotubes integrated with biological cultures and exposed to external stimuli has shown destruction of cells, thus indicating their use as synergistic agents of cell death. We propose to utilize the electrical properties of multi-walled carbon nanotubes (MWCNTs) to synergistically enhance pulse electric field effect induced cell death.
Detection of cytogenetic abnormalities in sperm from infertile males undergoing intracytoplasmic sperm injection. New York: Humana Press.
- Stacey, M. Biochemical and histological differences between costal and articular cartilage.Chest Wall Deformities. Springer Verlag.
- Taylor, A. Malignant disease and variations in radiosensitivity in Ataxia Telangiectasia patients.Genetic Predisposition to Cancer. London: Chapman & Hall..
- Kolb, J., and Stacey, M. Subcellular Biological Effects of Nanosecond Pulsed Electric Fields.Plasma for Bio-decontamination, Medicine and Food Security,”. NATO Science for Peace and Security Series, Springer Publishing.
- Stacey, M. W., Dutta, D., Cao, W., Asmar, A., Elsayed-Ali, H., Kelly, Jr., R., and Beskok, A. (2013). Atomic force microscopy characterization of collagen ’nanostraws’ in human costal cartilage. Micron, 44, (pp. 483-7).
- Stacey, M. W., Sabuncu, A. C., and Beskok, A. (2013). Dielectric Characterization of Costal Cartilage Chondrocytes. Biochimica Et Biophysica Acta
- Sabuncu, A. C., Stacey, M. W., and Beskok, A. (2013). Microfluidic Dielectric Spectroscopy of Costal Cartilage Chondrocytes. Biophysical Journal, 104 (2), (pp. 674a-674a).
- Stacey, M. W., Grubbs, J., Asmar, A., Pryor, J., Elsayed-Ali, H., Cao, W., Beskok, A., Dutta, D., Darby, D. A., Fecteau, A., Werner, A., and Kelly, Jr., R. E. (2012). Decorin expression, straw-like structure, and differentiation of human costal cartilage. Connect Tissue Res, 53 (5), (pp. 415-21).
- Basu, G., Kalluri, B. S., Sabuncu, A. C., Osgood, C. J., and Stacey, M. W. (2012). Enhanced Killing Effect of Nanosecond Pulse Electric Fields on PANC1 and Jurkat Cell Lines in the Presence of Tween 80. Journal of Membrane Biology, 245 (10), (pp. 611-616).
- Horth, L., Stacey, M., Benjamin, T., Segna, K., Proud, V. K., Nuss, D., and Kelly, Jr., R. E. (2012). Genetic analysis of inheritance of Pectus Excavatum. J. Pediatric Genet., 1 (3), (pp. 161-173).
- Sabuncu, A. C., Grubbs, J., Qian, S., Abdel-Fattah, T. M., Stacey, M. W., and Beskok, A. (2012). Probing nanoparticle interactions in cell culture media. Colloids Surf B Biointerfaces, 95, (pp. 96-102).
- Stacey, M. W., Osgood, C. J., Kalluri, B. S., Cao, W., Elsayed-Ali, H., and Abdel-Fattah, T. (2011). Nanosecond pulse electrical fields used in conjunction with multi-wall carbon nanotubes as a potential tumor treatment. Biomed Mater, 6 (1), (pp. 011002).
- Stacey, M. W., and Osgood, C. J. (2011). Nanosecond pulse electrical fields used in conjunction with mutli-wall carbon nanotubes as a potential tumor treatment.. Biomed Mater, 6, (pp. 011002).
- Stacey, M. W., Fox, P., Buescher, S., and Kolb, J. F. (2011). Nanosecond pulsed electric field induced cytoskeleton, nuclear membrane and telomere damage adversely impact cell survival. Bioelectrochemistry, 82 (2), (pp. 131-4).
- Sabuncu, A. C., Kalluri, B. S., Qian, S., Stacey, M. W., and Beskok, A. (2010). Dispersion state and toxicity of mwCNTs in cell culture medium with different T80 concentrations. Colloids and Surfaces B-Biointerfaces, 78 (1), (pp. 36-43).
- Stacey, M. W., Neumann, S. A., Dooley, A., Segna, K., Kelly, R. E., Nuss, D., Kuhn, A. M., Goretsky, M. J., Fecteau, A. H., Pastor, A., and Proud, V. K. (2010). Variable number of tandem repeat polymorphisms (VNTRs) in the ACAN gene associated with pectus excavatum. Clinical Genetics, 78 (5), (pp. 502-4).
- Kolb, J. F., Mohamed, A. A., Price, R. O., Swanson, R. J., Bowman, A., Chiavarini, R. L., Stacey, M. W., and Schoenbach, K. H. (2008). Cold atmospheric pressure air plasma jet for medical applications. Applied Physics Letters, 92 (24),
- Osgood, C. J., Schoenbach, K. H., Hargrave, B., Joshi, R., Kolb, J. F., Nuccitelli, R., Pakhomov, A., Stacey, M. W., Swanson, R. J., White, J., Xiao, S., and Zhang, J. (2007). Bioelectric effects of intense nanosecond pulses. IEEE Transact Dielectrics Electrical Insul, 14, (pp. 1088-1109).
- Sun, Y., Xiao, S., White, J. A., Kolb, J. F., Stacey, M. W., and Schoenbach, K. H. (2007). Compact, Nanosecond, High Repetition-Rate, Pulse Generator for Bioelectric Studies. IEEE Trans. Dielectrics and Electrical Insulation, 14 (4),
- Creswick, H. A., Stacey, M. W., Kelly, Jr., R. E., Gustin, T., Nuss, D., Harvey, R. H., Goretsky, M. J., Vasser, E., Welch, J. C., Mitchell, K., and Proud, V. K. (2006). Family study of the inheritance of pectus excavatum. Journal of Pediatric Surgery, 41 (10), (pp. 1699-703).
- Sutton, J. F., Stacey, M. W., Kearns, W. G., Rieg, T. S., Young, N. S., and Liu, J. M. (2004). Increased risk for aplastic anemia and myelodysplastic syndrome in individuals lacking glutathione S-transferase genes. Pediatr Blood Cancer, 42 (2), (pp. 122-6).
- Schoenbach, K. H., Joshi, R., Kolb, J. F., Chen, N., Stacey, M. W., Blackmore, P. F., Buescher, E. S., and Beebe, S. J. (2004). Ultrashort electrical pulses open a new gateway into biological cells. Proceedings of the Ieee, 92 (7), (pp. 1122-1137).
- Stacey, M. W., Stickley, J., Fox, P., Statler, V., Schoenbach, K. H., Beebe, S. J., and Buescher, S. (2003). Differential effects in cells exposed to ultra-short, high intensity electric fields: cell survival, DNA damage, and cell cycle analysis. Mutat Res, 542 (1-2), (pp. 65-75).
- Watters, A. D., Stacey, M. W., and Bartlett, J. (2002). A modified nick translation method used with FISH that produces reliable results with archival tissue sections. Molecular Biotechnology, 20 (3), (pp. 257-60).
- Aridgides, L. J., Stacey, M. W., Brihn, L., Scott, D., and Osgood, C. J. (2002). Fluorescence in situ hybridization on sperm using alkaline denaturation. Biotechniques, 33 (2), (pp. 266-7).
- Watters, A. D., Stacey, M. W., Going, J. J., Grigor, K. M., Cooke, T. G., Sim, E., and Bartlett, J. M. (2001). Genetic aberrations of NAT2 and chromosome 8: Their association with progression in transitional cell carcinoma of the urinary bladder. Urologia Internationalis, 67 (3), (pp. 235-239).
- Pfeffer, J., Pang, M. G., Hoegerman, S. F., Osgood, C. J., Stacey, M. W., Mayer, J., Oehninger, S., and Kearns, W. G. (1999). Aneuploidy frequencies in semen fractions from ten oligoasthenoteratozoospermic patients donating sperm for intracytoplasmic sperm injection. Fertility and Sterility, 72 (3), (pp. 472-478).
- Stacey, M. W., Matas, N., Drake, M., Payton, M., Fakis, G., Greenland, J., and Sim, E. (1999). Arylamine N-acetyltransferase type 2 (NAT2), chromosome 8 aneuploidy, and identification of a novel NAT1 cosmid clone: an investigation in bladder cancer by interphase FISH. Genes Chromosomes Cancer, 25 (4), (pp. 376-83).
- Thygesen, P., Risch, A., Stacey, M. W., Fakis, G., Takle, L., Knowles, M., and Sim, E. (1999). Genes for human arylamine N-acetyltransferases in relation to loss of the short arm of chromosome 8 in bladder cancer. Pharmacogenetics, 9 (1), (pp. 1-8).
- Stacey, M. W., Wang, J., Byrd, R. L., Liu, J. M., and Kearns, W. G. (1999). Nuclear receptor co-repressor gene localizes to 17p11.2, a frequently deleted band in malignant disorders. Genes Chromosomes Cancer, 25 (2), (pp. 191-3).
- Stacey, M. W., Payton, M., Fakis, G., Pope, J., and Sim, E. (1998). Characterisation of arylamine N-acetyltransferase in the urothelial cell line RT112. Pathogenesis, 1 (2), (pp. 99-105).
- Watters, A. D., Stacey, M. W., Going, J. J., Grigor, K. M., Cooke, T. G., Sim, E., and Barlett, J. M. (1998). Genetic alterations of N-acetyl transferase in transitional cell carcinoma of the bladder. British Journal of Cancer, 78 (2), (pp. 154-154).
- Stacey, M. W., Barlow, A., and Hulten, M. (1997). Human T-cell receptor zeta chain gene Map position 1q23.1. Chromosome Res, 5 (4), (pp. 279).
- Matas, N., Thygesen, P., Stacey, M. W., Risch, A., and Sim, E. (1997). Mapping AAC1, AAC2 and AACP, the genes for arylamine N-acetyltransferases, carcinogen metabolising enzymes on human chromosome 8p22, a region frequently deleted in tumours. Cytogenet Cell Genet, 77 (3-4), (pp. 290-5).
- Metcalfe, J. A., Parkhill, J., Campbell, L., Stacey, M. W., Biggs, P., Byrd, P. J., and Taylor, A. M. (1996). Accelerated telomere shortening in ataxia telangiectasia. Nat Genet, 13 (3), (pp. 350-3).
- Stacey, M. W., Thygesen, P., Stanley, L., Matas, N., Risch, A., and Sim, E. (1996). Arylamine N-acetyltransferase as a potential biomarker in bladder cancer: Fluorescent in situ hybridization and immunohistochemistry studies. Biomarkers, 1 (1), (pp. 55-61).
- Hulten, M. A., Stacey, M. W., and Armstrong, S. J. (1995). Does junk DNA regulate gene expression in humans?. Clin Mol Pathol, 48 (3), (pp. M118-23).
- Stacey, M. W., Bennett, M. S., and Hulten, M. (1995). FISH analysis on spontaneously arising micronuclei in the ICF syndrome. J Med Genet, 32 (7), (pp. 502-8).
- Hernandez, D., McConville, C. M., Stacey, M. W., Woods, C. G., Brown, M. M., Shutt, P., Rysiecki, G., and Taylor, A. M. (1993). A family showing no evidence of linkage between the ataxia telangiectasia gene and chromosome 11q22-23. J Med Genet, 30 (2), (pp. 135-40).
- Moss, C., Larkins, S., Stacey, M. W., Blight, A., Farndon, P. A., and Davison, E. V. (1993). Epidermal mosaicism and Blaschko’s lines. J Med Genet, 30 (9), (pp. 752-5).
- Taylor, A. M., Lowe, P. A., Stacey, M. W., Thick, J., Campbell, L., Beatty, D., Biggs, P., and Formstone, C. J. (1992). Development of T-cell leukaemia in an ataxia telangiectasia patient following clonal selection in t(X;14)-containing lymphocytes. Leukemia, 6 (9), (pp. 961-6).
- McKeown, C. M., Waters, J. J., Stacey, M. W., Newman, B. F., Cardy, D. L., and Hulten, M. (1992). Rapid interphase FISH diagnosis of trisomy 18 on blood smears. Lancet, 340 (8817), (pp. 495).
- Stacey, M. W., Gallimore, P. H., McConville, C., and Taylor, A. M. (1990). Rearrangement of the same chromosome regions in different SV40 transformed human skin keratinocyte lines is associated with tumourigenicity. Oncogene, 5 (5), (pp. 727-39).
- Stacey, M. W., Thacker, S., and Taylor, A. M. (1989). Cultured skin keratinocytes from both normal individuals and basal cell naevus syndrome patients are more resistant to gamma-rays and UV light compared with cultured skin fibroblasts. Int J Radiat Biol, 56 (1), (pp. 45-58).
- Taylor, A. M., Flude, E., Laher, B., Stacey, M. W., McKay, E., Watt, J., Green, S. H., and Harding, A. E. (1987). Variant forms of ataxia telangiectasia. J Med Genet, 24 (11), (pp. 669-77).
Conference Name: Am. Soc. Matrix Biol.
Location: Charleston SC
Date: October 25, 2010
Contracts, Grants and Sponsored Research
Electrochemotherapy and nanomedicine combine to enhance tumor cell death
Sponsoring Organization: CHRB
Date: July, 2009 - June, 2010
Investigators:Osgood, C. J.; Stacey, M. W.