CBE Seminar - David A. Dean, PhD, U. Rochester Medical School, Rochester, NY
- 09/16/2011 10:00 AM - 09/16/2011 11:00 AM
- IRP2 1st Floor Conference Room
- "The cell biology of gene delivery: what happens to DNA after electroporation"
"The cell biology of gene delivery: what happens to DNA after electroporation"
David A. Dean, PhD
Professor, Department of Pediatrics
University of Rochester
Friday, September 16, 2011
IRP#1 Conference Room, Second Floor
4111 Monarch Way (near Ted Constant Center)
Dr. Dean's Research:
Despite all of the hype regarding gene therapy, at present, gene therapy is a dream due to insufficient levels of gene transfer and expression at desired sites. One way to increase gene expression is to target more DNA to the cell nucleus. Since the nucleus is the site of transcription, without the movement of plasmids through the cytoplasm, translocation into the nucleus, and localization to the appropriate subnuclear domain, no gene expression, or "gene therapy" can take place. Ongoing projects in my laboratory are studying the mechanisms of cytoplasmic trafficking of plasmids along the cytoskeleton, plasmid nuclear entry, subnuclear organization, and exploiting what we learn to improve gene therapy. (www.urmc.rochester.edu)