CBE Seminar - Gintautas Saulis, PhD, Vytautas Magnus University, Kaunas, Lithuania
- 10/17/2011 10:00 AM - 10/17/2011 11:00 AM
- IRP2 1st Floor Conference Room
- "Electrochemical processes occurring during cell electromanipulation procedures and their consequences"
"Electrochemical processes occurring during cell electromanipulation procedures and their consequences"
Gintautas Saulis, PhD
Laboratory of Biophysics for Bionanotechnology and Medicine
Department of Biology, Faculty of Nature Sciences
Vytautas Magnus University, Kaunas, Lithuania
Friday, October 7, 2011, 10-11 AM
IRP2 First Floor Conference Room
4211 Monarch Way (near Ted Constant Center)
Dr. Saulis' Research:
During cell electromanipulation procedures, a cell suspension is exposed to high-voltage electric pulses and a strong electric current passes through the solution. At each electrode-solution interface, various electrochemical reactions occur. These may include the evolution of gas, the separation of substances, the dissolution of the electrode or the appearance of new substances in the solution. The processes of electrolysis lead to the changes of the temperature, pH, and the chemical composition of the experimental medium.
In this presentation, a short theoretical background of these electrochemical processes will be provided. The consequences of the primary and secondary electrochemical processes, which might be important for the understanding the results obtained as well as optimization of cell electromanipulation procedures, will be discussed to demonstrate that, when using high-voltage electrical pulses for electroporation of cells and tissues, scientists must keep in mind that the products generated due to electrochemical reactions can influence the biochemical processes taking part in their experimental systems.